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Chromogenic Endotoxin Quantification Kit for Accurate Bacterial Toxin Measurement

Endotoxins, also known as lipopolysaccharides (LPS), are toxic components of the outer membrane of Gram-negative bacteria. Accurate measurement of endotoxins is critical in pharmaceutical, medical device, and biotechnology industries to ensure product safety. The Chromogenic Endotoxin Quant Kit provides a reliable and sensitive method for quantifying bacterial endotoxins.

Why Choose the Chromogenic Endotoxin Quant Kit?

The Chromogenic Endotoxin Quant Kit utilizes a chromogenic assay based on the Limulus Amebocyte Lysate (LAL) principle. This method offers several advantages:

• High sensitivity – detects endotoxin levels as low as 0.005 EU/mL
• Wide dynamic range – measures endotoxin concentrations from 0.005 to 50 EU/mL

Keyword: Chromogenic Endotoxin Quant Kit

• Rapid results – provides quantitative data in approximately 60 minutes
• Simple procedure – requires minimal sample preparation
• Compatibility – works with various sample types including water, buffers, and biological fluids

How the Chromogenic Assay Works

The kit employs a two-step enzymatic reaction. First, endotoxins activate Factor C in the LAL reagent. This activated enzyme then cleaves a synthetic chromogenic substrate, releasing p-nitroaniline (pNA). The intensity of the yellow color produced is directly proportional to the endotoxin concentration in the sample, which can be measured spectrophotometrically at 405 nm.

Applications of Endotoxin Testing

The Chromogenic Endotoxin Quant Kit has numerous applications across different industries:

• Pharmaceutical manufacturing – testing raw materials, water for injection, and final products
• Medical device production – evaluating device safety before clinical use
• Biotechnology research – monitoring endotoxin levels in cell culture media and recombinant proteins
• Clinical diagnostics – detecting endotoxemia in patient samples

Key Features of the Kit

The Chromogenic Endotoxin Quant Kit includes all necessary components for accurate endotoxin measurement:

• Lyophilized LAL reagent with chromogenic substrate
• Endotoxin standard (0.5 EU/vial)
• LAL reagent water (LRW)
• Detailed protocol and data analysis instructions

Conclusion

The Chromogenic Endotoxin Quant Kit provides researchers and quality control professionals with a precise, reproducible method for bacterial endotoxin detection. Its chromogenic approach offers superior sensitivity and quantitative accuracy compared to traditional gel-clot methods. By implementing this kit in your endotoxin testing workflow, you can ensure compliance with regulatory requirements while maintaining high standards of product safety.